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column  (TaKaRa)


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  • 96

    Structured Review

    TaKaRa column
    Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+clean+up+columns/NucleoSpin+Gel+and+PCR+Clean-Up/pmc13128445-246-40-43
    Average 96 stars, based on 912 article reviews
    column - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP.
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Purification:

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Structure of the PCNA unloader Elg1-RFC
    Article Snippet: .. The reaction was then purified using Macherey-Nagel NucleoSpin Gel and PCR Clean-up columns (Takara Bio, USA, ref no. 740609.10) eluted in 5 mM tris-HCl (pH 8.5) and 1 mM EDTA. ..

    Article Title: Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis.
    Article Snippet: INTRODUCTION: DNA polymerases (Pols), such as the leading-strand Pol (Pole) and the laggingstrand Pol (Pold) function within a holoenzyme context with the proliferating cell nuclear antigen (PCNA)DNA-sliding clamp for activity and processivity.. The PCNA clamp is a closed ring andneeds to be opened and closed aroundDNA by a clamp loader that uses ATP.. It has been assumed that the PCNAs on both leadingand lagging-strand DNA are loaded by the replication factor C (RFC), a pentameric ATPase complex composed of Rfc1 to Rfc5.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP.
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Light-switchable transcription factors obtained by direct screening in mammalian cells
    Article Snippet: .. The product was purified and digested with HindIII and BglII followed by purification with NucleoSpin Gel and PCR Clean-up Columns (Takara, #740609). ..

    Article Title: Structure of the PCNA unloader Elg1-RFC.
    Article Snippet: .. The reaction was then purified using Macherey- Nagel NucleoSpin Gel and PCR Clean- up columns (Takara Bio, USA, ref no. 740609.10) eluted in 5 mM tris- HCl (pH 8.5) and 1 mM EDTA. ..

    Polymerase Chain Reaction:

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA binding protein-dependent manner with chimeric eCLIP
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Structure of the PCNA unloader Elg1-RFC
    Article Snippet: .. The reaction was then purified using Macherey-Nagel NucleoSpin Gel and PCR Clean-up columns (Takara Bio, USA, ref no. 740609.10) eluted in 5 mM tris-HCl (pH 8.5) and 1 mM EDTA. ..

    Article Title: Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis.
    Article Snippet: INTRODUCTION: DNA polymerases (Pols), such as the leading-strand Pol (Pole) and the laggingstrand Pol (Pold) function within a holoenzyme context with the proliferating cell nuclear antigen (PCNA)DNA-sliding clamp for activity and processivity.. The PCNA clamp is a closed ring andneeds to be opened and closed aroundDNA by a clamp loader that uses ATP.. It has been assumed that the PCNAs on both leadingand lagging-strand DNA are loaded by the replication factor C (RFC), a pentameric ATPase complex composed of Rfc1 to Rfc5.

    Article Title: Mapping snoRNA-target RNA interactions in an RNA-binding protein-dependent manner with chimeric eCLIP.
    Article Snippet: End-repaired RNA was purified using RNA Clean & Concentrator-5 columns (Zymo). .. Illumina libraries were then prepared using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs) and purified on NucleoSpin® Gel and PCR Clean-up columns (Takara Bio). .. Illumina sequencing of the libraries was performed on HiSeq (50 bp SE) or NovaSeq (SE100 or PE100) platforms.

    Article Title: Light-switchable transcription factors obtained by direct screening in mammalian cells
    Article Snippet: .. The product was purified and digested with HindIII and BglII followed by purification with NucleoSpin Gel and PCR Clean-up Columns (Takara, #740609). ..

    Article Title: Structure of the PCNA unloader Elg1-RFC.
    Article Snippet: .. The reaction was then purified using Macherey- Nagel NucleoSpin Gel and PCR Clean- up columns (Takara Bio, USA, ref no. 740609.10) eluted in 5 mM tris- HCl (pH 8.5) and 1 mM EDTA. ..

    other:

    Article Title: Multimodal control of Cas13 activity through domain insertion at an allosteric hotspot
    Article Snippet: UniRapR sequence was cloned from pUSE-Src-YF-UniRapR-mCerulean-myc (Addgene #45381) 31 .



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